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January 25, 2024

If the documents are not clear here is the exact content in them:

Genome Editing with CRISPR

Instructions:

Use the website below to answer the following questions;

https://www.broadinstitute.org/what-broad/areas-focus/project-spotlight/crispr

Watch the YouTube video Genome Editing with CRISPR-Cas9.

Click on the link to the right of the screen “Questions and Answers about CRISPR” and answer the following questions.

1. What is “CRISPR”?

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2. Where do CRISPRs come from?

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3. How does the system work?

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4. How does CRISPR-Cas9 compare to other genome editing tools?

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5. Read the timeline (link to the right) and highlight the important discoveries that ultimately led up to its ongoing development into a genome editing tool under the following headings.

Discovery of CRISPR and its function

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Discovery of Cas9 and PAM

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Hypothetical scheme of adaptive immunity

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Experimental demonstration of adaptive immunity

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Spacer sequences are transcribed into guide RNAs

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CRISPR acts on DNA targets

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Cas9 cleaves target DNA

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Discovery of tracrRNA for Cas9 system

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CRISPR systems can function heterologously in other species

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Biochemical characterization of Cas9-mediated cleavage

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CRISPR-Cas9 harnessed for genome editing

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6. Use the picture to the right to answer the following questions.

a. What is a phage?

b. What is the phage doing?

c. Describe the mechanism for removing the invade DNA.

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