There are two main types of mutations: point mutation and frameshift mutations. In each of the following DNA sequences, you will use the mRNA and amino acid sequences to identify the mutation that occurred and the effects of each, if any. Look and analyze carefully.
Question 1
Original DNA Sequence: T A C A C C T T G G C G A C G A C T
mRNA Sequence: AUG UGG ACC CGC UGC UGA
Amino Acid Sequence: Met-Thr-Asn-Arg-Cys-stop
Question 2
Mutated DNA Sequence #1: A C A T C T T G G C G A C GA C T
What’s the mRNA sequence? (Indicate the change) UGU-AGA-ACC-GCU-GCU-GA
What will be the amino acid sequence? Cys-Arg-Thr-Ala-Ala
Will there likely be effects?______________________
What kind of mutation is this?______________________
Question 3
Mutated DNA Sequence#2: T A C G A C C T T G G C G A C G A C T
What’s the mRNA sequence? (Indicate the change) AUG-CUG-GAA-CCG-CUG-A
What will be the amino acid sequence? Met-Leu-Glu-Pro-Leu-
Will there likely be effects? _______________________________________________
What kind of mutation is this? ____________________________________________
Question 4
Mutated DNA Sequence#3: T A C A C C T T G G C G A C T A C T
What’s the mRNA sequence? (Indicate the change) AGU-UGG-AAC-CGC-UGA-UGA
What will be the amino acid sequence? Met-Trp-Asn-Arg-stop-stop
Will there likely be effects? _______________________________________________
What kind of mutation is this? ____________________________________________
Question 5
Mutated DNA Sequence #4: T A C A C C T T G G G A C G A C T
What kind of mutation is this? ____________________________________________
What will be the corresponding mRNA sequence? AUG-UGG-AAC-CCU-GCU-GA
What will be the amino acid sequence? Met-Trp-Asn-Pro-Ala-
Will there likely be effects? _______________________________________________
What kind of mutation is this? ____________________________________________
