Lab Worksheet on Histology.
Observation of different tissues of the body
- Obtain the preserved slide of tissue. Your instructor will you a list of tissues to be observed and prepared slides.
- Observe the slide under 10X, and 40X objective lenses.
Don’t forget to use oil with 100X lens only. After completing the observation, clean the oil immersion lens with lens paper to remove oil. Be careful to not contaminate other lenses (4X, 10X, 40X) with oil.
- Sketch and describe your observations made using 40X and 100X lenses. Make sure to note as many details as possible. (Please note that your ability for artwork is not a problem, but the effort made to draw and describe in detail is good.
- Repeat the steps for another slide of a tissue.
Observation: Simple Squamous Epithelium
Observation: Stratified Squamous Epithelium
Observation: Simple Cuboidal Epithelium
Observation: Simple Columnar
Observation: Pseudostratified Columnar Epithelium (with or without cilia)
Observation: Transitional Epithelium
Observation: Adipose CT
Observation: Blood
Observation: Skeletal Muscle
Observation: Smooth Muscle
Observation: Cardiac Muscle
Observation: Nervous Tissue
Complete the missing information on each Histology Table.
| Specific Epithelial Tissue | Characteristics and function | Location |
Lungs Kidneys Linings of blood vessels | ||
| Simple Cuboidal | Kidneys (tubules) Exocrine glands (small ducts) | |
| Simple Columnar | Ciliated: Bronchi Uterus Uterine (Fallopian) tubes Non-ciliated: Gastrointestinal (GI) tract | |
| Many layers provide mechanical protection and abrasion resistance. | Skin Mouth Esophagus Vagina | |
| Stratified Cuboidal | Large exocrine glands (mammary, sweat, salivary) | |
| Stratified Columnar | Protection and secretion |
|
| Usually ciliated. Secretion and propulsion of mucus | Trachea Male reproductive ducts | |
| Transitional |
|
| Specific Tissue | Characteristics and function | Location |
| 1. Loose Connective Tissue | Protein fibers less densely organized | |
| Areolar (fascia) CT | Lamina propria of mucus membranes Wraps and cushions most organs
| |
| Hypodermis Abdomen Breasts | |
| Reticular CT | Contains primarily lymphocytes, mast cells, and stem cells. Protein fibers dominated by reticulin, a highly branched support structure. | |
| 2. Dense Connective Tissue | Protein fibers arrange in dense fibers | |
| Dense Regular CT | Cell population dominated by fibroblasts. Protein fibers dominated by collagen arranged in long parallel bundles. Supplies great strength when force is applied along a single axis. | |
| Cell population dominated by fibroblasts. Protein fibers dominated by collagen arranged in many small bundles oriented in many directions. Supplies strength and elasticity when force is applied in many different directions. | Dermis (of skin) Submucosa of GI tract Joint capusles Capsules of many organs (kidney, etc.) Periosteum | |
| 3. Supporting Connective Tissue | ||
| Cartilage | Chondrocytes housed in small openings (lacunae) within a rubbery matrix of polysaccharide (chondroitin sulfate) and proteoglycans. Completely avascular | |
| Cushioning, support, reinforcement. Matrix relatively even and smooth. | Ends of long bones Ribs Trachea | |
| Elastic Cartilage | Ear Epiglottis Pharyngotympanic tube | |
| Fibrous Cartilage | More collagen fibers for high tensile strength and shock absorption. Matrix contains many parallel collagen fibers. |
|
| Fluid Connective Tissue | ||
| Red and white blood cells found within a fluid matrix containing primarily albumin protein. | Within blood vessels | |
| Lymphocytes found within a fluid matrix containing small amounts of dissolved protein. | Within lymphatic vessels |
| Specific Tissue | Characteristics and function | Location |
| Skeletal Muscle | ||
Walls of blood vessels Viscera of digestive organs Iris of eye Involuntary sphincters- (urethra and anus) | ||
| Cardiac Muscle |
| Specific Tissue: | Characteristics and function | Location |
| Nervous | Specialized for communication through use of electrical and chemical signals. Consists of neurons, the main cell type, and neuroglial cells, which support the neuron. |
