. If an objective lens has 50x magnification, and the ocular 10x, what is the total magnification of the image?
2B. If you move the slide to the right, which way does the image move?
2C. If the diameter of the field of view under 50x total magnification is 5 mm, then the diameter of the field of view under 400x magnification is how many mm?
2D. What is the proper way to store a microscope?
2E. Estimate the size of the object if the field of view diameter is 2.5 mm.
3. Identify the stages of mitosis indicated in the picture. (5 points)
a.
b.
c.
d.
e.
3B. Are these animal or plant cells? How do you know? (2 points)
4. Answer the following questions about mitosis. (7 points)
4A. There are three stages to interphase. What are they? In which one is DNA replicated?
4B. Leaving out interphase, which stage does the cell spend the most time in?
4C. What is the splitting of the cytoplasm called?
4D. If a cell started with 40 chromosomes, how many does it have at the end of mitosis?
5. In corn, starchy (S) is dominant to sweet (s). A true breed sweet plant was crossed with a true breed starchy plant to produce an F1 generation. The F1’s were crossed to produce an F2 generation.
Diagram the cross giving the genotypes and phenotypes of all 3 generations. (10 points)
6. In corn, starchy (S) is dominant to sweet (s), and purple kernels (P) are dominant over yellow (p) A true breed sweet, purple plant was crossed with a true breed starchy, yellow plant to produce an F1 generation. The F1’s were crossed to produce an F2 generation.
Diagram the cross giving the genotypes and phenotypes of all 3 generations. (12 points)
7. The F2 results of the cross in question 6 were 927 starchy, purple, 290 starchy, yellow, 316 sweet, purple, and 67 sweet, yellow. Determine if those results fit what is expected. Show all work. (10 points)
8. Describe a procedure how to amplify a specific region of DNA. Be sure in your description you tell how to specify the region, what reagents are needed for the whole process, and all the steps, with some general specifications for each step. (12 points)
9. In making a new mutation, I introduced a new restriction enzyme site into the gene to make identifying the mutation easier. The original gene was 1000 base pairs. The mutation was cut by the restriction enzyme one time, leaving pieces of 700 and 300 base pairs. Three different samples were digested and the DNA pieces run on an agarose gel using electrophoresis. The results are below.
Identify which sample is correct (having only the mutation), explaining why it is correct and the others are not. Lane 1 are the markers, Lanes 2, 3, and 4 are different samples. (9 points)
