Define PCR.
List all that is needed in order to amplify a small sample of DNA through PCR.
Why is it necessary to have a aprimer present on each side of the DNA segment to amplified?
State and describe the 3 steps for PCR. Include the temperature at which each step occurs.
A single stranded DNA has the base sequence: 5′- ATTGGCACC -3′. Which of the following DNA sequences shown below will hybridize with this DNA strand.
5′ – TAACCGTGG -3′
5′ – UAACCGACC -3′
3′ – TAACCGTGG – 5′
3′ – UAACCGTGG – 5′
What is the difference between an intron and an exon?
Explain how agarose gel electrophoresis separates DNA fragments. Why does a smaller DNA fragment travel faster than a larger one?
What is the genotype for the Alu insert in your PV92 region?
