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Define PCR. List all that is needed in order to amplify a small…

Define PCR.

List all that is needed in order to amplify a small sample of DNA through PCR. 

Why is it necessary to have a aprimer present on each side of the DNA segment to amplified?

State and describe the 3 steps for PCR. Include the temperature at which each step occurs. 

 

A single stranded DNA has the base sequence: 5′- ATTGGCACC -3′. Which of the following DNA sequences shown below will hybridize with this DNA strand. 

5′ – TAACCGTGG -3′

5′ – UAACCGACC -3′

3′ – TAACCGTGG – 5′

3′ – UAACCGTGG – 5′

 

What is the difference between an intron and an exon?

Explain how agarose gel electrophoresis separates DNA fragments. Why does a smaller DNA fragment travel faster than a larger one? 

What is the genotype for the Alu insert in your PV92 region? 

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